Abstract:The method of fingerprinting and content determination of multi-characteristic components of Adiantum capillus-junonis Rupr.was established and applied to the quality control of A. capillus-junonis Rupr.in different origin.Method:The chromatographic condition was set up on the SHISEIDO CAPCELL PAK C18 chromatographic column (4.6 mm×250 mm,5 μm) by gradient elution of methanol-0.05% phosphoric acid,the monitoring wavelength was 280 nm,detection temperature was 35 ℃.The technique was used to establish a HPLC-DAD analysis method for the analysis of fingerprint and multi-characteristic components of A. capillus-junonis Rupr.And similarity software and chemometrics analysis was used to quality evaluation of multi-batch medicinal materials.There were 13 common peaks in this method,and 2 were identified as quercetin-3-O-β-D-glucuronide and kaempferol 3-O-rutinoside respectively,similarity ranged from 0.558 to 0.901,combined with cluster analysis and principal component analysis,13 common peaks were evaluated by orthogonal partial least squares discriminant analysis,and the contents of quercetin-3-O-β-D-glucuronide and kaempferol 3-O-rutinoside were determined in the range of 0.11~4.35 mg/g and 0.15~10.35 mg/g,respectively.It can be seen from the results that there is a great difference in the A. capillus-junonis Rupr.medicinal materials from different habitats.The HPLC-DAD analysis method for fingerprinting and content determination of multi-characteristic components of A. capillus-junonis Rupr.can more comprehensively and accurately reflect the contribution of the overall chemical composition of A. capillus-junonis Rupr.and the content of various characteristic components.It lays a foundation for improving the quality control method of A. capillus-junonis.